MOLTOX FT® E. coli Mutagenicity Assay Kit

MOLTOX FT® tests measure the ability of test material treatments to induce reversion of histidine-requiring Salmonella typhimurium strains or Escherichia coli tryptophan auxotrophs to their respective prototrophic conditions. The bacterial strains used in these assays are identical to those used in conventional plate incorporation assays as described by Mortlemans & Riccio, Mutat. Res. 455: 61, 2000. The experimental design used in the MOLTOX FT® tests is based on Gatehouses’s adaption of the design reported by Luria & Delbruck, Mutat. Res. 53: 289, 1978 and Genetics, 28: 491, 1943. The MOLTOX FT® E. coli Mutagenicity Assay Kit consists of materials to perform a microtiter fluctuation test using Escherichia coli WP2 (trp, pKM101) and WP2 (trp, uvrA). This assay includes sufficient components for 1 test material in triplicate analysis of 6 concentrations, positive and negative controls, with and without S9.